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KMID : 0358619960300010077
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Korean Journal of Physiology
1996 Volume.30 No. 1 p.77 ~ p.83
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Effects of Platelet-Activating Factor on Tumor Necrosis Production by Muramyl Dipeptide- or Silica-Stimulated Alveolar Macrophages
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Lee Ji-Hee
Hah Jong-Sik
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Abstract
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Platelet-activating factor(PAF) is a phospholipid mediator of pulmonary inflammation, and immunologic reaction. In this study, the role of PAF on tumor necrosis factor production by rat alveolar macrophages(AM) was examined. When PAF alone was added to AM culture, production was not significantly increased above the resting level. In contrast, the combined addition of PAF and muramyl dipeptide(MDP) to AM cultures markedly enhanced production with 8.2 fold increase compared with AM culture in resting state. This potentiative effect was 313% above the sum of the separate effects of PAF and MDP. To characterize MDP effects on production, the dose-response of AM cultured with various concentrations of MDP was tested. High level of MDP could not significantly enhance the potentiation effect on production compared with AM cultures with low level of MDP , i.e. 112.5% vs 107.8%, respectively when M of PAF was simultaneously added to the cell culture. These data support that the potentiation of TNF. g production in AM culture is mediated by PAF rather than MDf It was also evaluated whether the similar result was obtained in silica, respirable toxic particle-treated AM culture. production was also significantly enhanced in the PAF and silica -added cell cultures with 4.7 fold above the value of silica alone-stimulated cells. These results indicate that PAF can potentiate production by MDP-or silica- stimulated AM and suggest that PAF may play a potent role in lung inflammation and disease associated with microbe and occupational dust exposures.
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KEYWORD
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PAF, Alveolar macrophages, MDP, Silica
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